Review



c pdsred2 c1 1nls d pdsred2 c1 s100a10 wt e pdsred2 c1 s100a10 1nls f control g pdsred2 c1 wt  (TaKaRa)


Bioz Verified Symbol TaKaRa is a verified supplier
Bioz Manufacturer Symbol TaKaRa manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    TaKaRa c pdsred2 c1 1nls d pdsred2 c1 s100a10 wt e pdsred2 c1 s100a10 1nls f control g pdsred2 c1 wt
    Map and MCS region of <t>pDsRed2-C1-wt.</t> a Vector of pDsRed2-C1-wt (Clontech), b pDsRed2-C1-wt MCS region (Clontech). c MCS regions of pDsRed2-C1-wt and <t>pDsRed2-C1-1NLS.</t> The NLS sequence was inserted between positions 1335 and 1339 in the sequence of pDsRed2-C1-wt (Clontech), and the CCG sequence was mutated to the CGC sequence. (a) was made by plasMapper 2.0 (https://wishart.biology.ualberta.ca/PlasMapper/index.html). For more information of pDsRed2-C1-wt, visit Clontech site (https://www.takarabio.com/assets/documents/Vector%20Documents/PT3603-5.pdf)
    C Pdsred2 C1 1nls D Pdsred2 C1 S100a10 Wt E Pdsred2 C1 S100a10 1nls F Control G Pdsred2 C1 Wt, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 288 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdsred2+f+vector/pDsRed2-C1+Vector/pmc06534648-171-22-32
    Average 95 stars, based on 288 article reviews
    c pdsred2 c1 1nls d pdsred2 c1 s100a10 wt e pdsred2 c1 s100a10 1nls f control g pdsred2 c1 wt - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Development of a novel DsRed-NLS vector with a monopartite classical nuclear localization signal"

    Article Title: Development of a novel DsRed-NLS vector with a monopartite classical nuclear localization signal

    Journal: 3 Biotech

    doi: 10.1007/s13205-019-1770-0

    Map and MCS region of pDsRed2-C1-wt. a Vector of pDsRed2-C1-wt (Clontech), b pDsRed2-C1-wt MCS region (Clontech). c MCS regions of pDsRed2-C1-wt and pDsRed2-C1-1NLS. The NLS sequence was inserted between positions 1335 and 1339 in the sequence of pDsRed2-C1-wt (Clontech), and the CCG sequence was mutated to the CGC sequence. (a) was made by plasMapper 2.0 (https://wishart.biology.ualberta.ca/PlasMapper/index.html). For more information of pDsRed2-C1-wt, visit Clontech site (https://www.takarabio.com/assets/documents/Vector%20Documents/PT3603-5.pdf)
    Figure Legend Snippet: Map and MCS region of pDsRed2-C1-wt. a Vector of pDsRed2-C1-wt (Clontech), b pDsRed2-C1-wt MCS region (Clontech). c MCS regions of pDsRed2-C1-wt and pDsRed2-C1-1NLS. The NLS sequence was inserted between positions 1335 and 1339 in the sequence of pDsRed2-C1-wt (Clontech), and the CCG sequence was mutated to the CGC sequence. (a) was made by plasMapper 2.0 (https://wishart.biology.ualberta.ca/PlasMapper/index.html). For more information of pDsRed2-C1-wt, visit Clontech site (https://www.takarabio.com/assets/documents/Vector%20Documents/PT3603-5.pdf)

    Techniques Used: Plasmid Preparation, Sequencing

    Primers used for subcloning and site-directed mutagenesis
    Figure Legend Snippet: Primers used for subcloning and site-directed mutagenesis

    Techniques Used: Subcloning, Construct

    Confocal microscopy experiments. Vectors transfected in COS1 (a–e) and COS7 (f–j) cells. a Control b pDsRed2-C1-wt (Clontech) c pDsRed2-C1-1NLS d pDsRed2-C1-S100A10-wt e pDsRed2-C1-S100A10-1NLS f control g pDsRed2-C1-wt (Clontech) h pDsRed2-C1-1NLS i pDsRed2-C1-S100A10-wt j pDsRed2-C1-S100A10-1NLS. Controls (a) and (f) correspond to non-treated COS1 and COS7 cells
    Figure Legend Snippet: Confocal microscopy experiments. Vectors transfected in COS1 (a–e) and COS7 (f–j) cells. a Control b pDsRed2-C1-wt (Clontech) c pDsRed2-C1-1NLS d pDsRed2-C1-S100A10-wt e pDsRed2-C1-S100A10-1NLS f control g pDsRed2-C1-wt (Clontech) h pDsRed2-C1-1NLS i pDsRed2-C1-S100A10-wt j pDsRed2-C1-S100A10-1NLS. Controls (a) and (f) correspond to non-treated COS1 and COS7 cells

    Techniques Used: Confocal Microscopy, Transfection

    Fluorescence density analysis. Vectors transfected in COS1 (a–e) and COS7 (f–j) cells. a Control b pDsRed2-C1-wt (Clontech) c pDsRed2-C1-1NLS d pDsRed2-C1-S100A10-wt e pDsRed2-C1-S100A10-1NLS f control g pDsRed2-C1-wt (Clontech) h pDsRed2-C1-1NLS i pDsRed2-C1-S100A10-wt j pDsRed2-C1-S100A10-1NLS. Blue line represents the fluorescence intensity in the nucleus and red represents the fluorescence intensity of DsRed. Controls (a) and (f) correspond to non-treated COS1 and COS7 cells
    Figure Legend Snippet: Fluorescence density analysis. Vectors transfected in COS1 (a–e) and COS7 (f–j) cells. a Control b pDsRed2-C1-wt (Clontech) c pDsRed2-C1-1NLS d pDsRed2-C1-S100A10-wt e pDsRed2-C1-S100A10-1NLS f control g pDsRed2-C1-wt (Clontech) h pDsRed2-C1-1NLS i pDsRed2-C1-S100A10-wt j pDsRed2-C1-S100A10-1NLS. Blue line represents the fluorescence intensity in the nucleus and red represents the fluorescence intensity of DsRed. Controls (a) and (f) correspond to non-treated COS1 and COS7 cells

    Techniques Used: Fluorescence, Transfection

    Immunoprecipitation of modified pDsRed2-C1-1NLS and pDsRed2-C1-S100A10-1NLS. Immunoprecipitation was performed using COS1 and COS7 cell lines. pDsRed2-C1-wt (Clontech), pDsRed2-C1-1NLS, pDsRed2-C1-S100A10-wt, and pDsRed2-C1-S100A10-1NLS vectors were transfected into each cell line for 24 h. Cells were lysed after transfection and immunoprecipitation was performed in the lysate using DsRed, karyopherin α2/β2 antibodies, and protein A/G plus-agarose (Santa Cruz). Samples that transfected with pDsRed2-C1-wt (Clontech), pDsRed2-C1-1NLS, pDsRed2-C1-S100A10-wt, pDsRed2-C1-S100A10-1NLS were identified using antibodies that detected karyopherin α2/β2 protein. Samples evaluated for karyopherin α2/β2 were confirmed by western blotting using antibodies for DsRed protein. Anti-β-actin (C4) antibody (Santa Cruz) was used to detect a reference protein in whole cell lysates prior to immunoprecipitation
    Figure Legend Snippet: Immunoprecipitation of modified pDsRed2-C1-1NLS and pDsRed2-C1-S100A10-1NLS. Immunoprecipitation was performed using COS1 and COS7 cell lines. pDsRed2-C1-wt (Clontech), pDsRed2-C1-1NLS, pDsRed2-C1-S100A10-wt, and pDsRed2-C1-S100A10-1NLS vectors were transfected into each cell line for 24 h. Cells were lysed after transfection and immunoprecipitation was performed in the lysate using DsRed, karyopherin α2/β2 antibodies, and protein A/G plus-agarose (Santa Cruz). Samples that transfected with pDsRed2-C1-wt (Clontech), pDsRed2-C1-1NLS, pDsRed2-C1-S100A10-wt, pDsRed2-C1-S100A10-1NLS were identified using antibodies that detected karyopherin α2/β2 protein. Samples evaluated for karyopherin α2/β2 were confirmed by western blotting using antibodies for DsRed protein. Anti-β-actin (C4) antibody (Santa Cruz) was used to detect a reference protein in whole cell lysates prior to immunoprecipitation

    Techniques Used: Immunoprecipitation, Modification, Transfection, Western Blot

    Related Articles

    Cell Culture:

    Article Title: Brain-derived neurotrophic factor uses CREB and Egr3 to regulate NMDA receptor levels in cortical neurons
    Article Snippet: .. Primary neocortical cultured neurons were transfected with pDsRed2-F vector (Clontech) at DIV 7–8 to identify both plasma membranes and transfected neurons. ..

    Article Title: Brain-derived neurotrophic factor uses CREB and Egr3 to regulate NMDA receptor levels in cortical neurons
    Article Snippet: .. Fluorescent microscopy Primary neocortical cultured neurons were transfected with pDsRed2-F vector (Clontech) at DIV 7–8 to identify both plasma membranes and transfected neurons. ..

    Transfection:

    Article Title: Brain-derived neurotrophic factor uses CREB and Egr3 to regulate NMDA receptor levels in cortical neurons
    Article Snippet: .. Primary neocortical cultured neurons were transfected with pDsRed2-F vector (Clontech) at DIV 7–8 to identify both plasma membranes and transfected neurons. ..

    Article Title: Brain-derived neurotrophic factor uses CREB and Egr3 to regulate NMDA receptor levels in cortical neurons
    Article Snippet: .. Fluorescent microscopy Primary neocortical cultured neurons were transfected with pDsRed2-F vector (Clontech) at DIV 7–8 to identify both plasma membranes and transfected neurons. ..

    Plasmid Preparation:

    Article Title: Brain-derived neurotrophic factor uses CREB and Egr3 to regulate NMDA receptor levels in cortical neurons
    Article Snippet: .. Primary neocortical cultured neurons were transfected with pDsRed2-F vector (Clontech) at DIV 7–8 to identify both plasma membranes and transfected neurons. ..

    Article Title: Brain-derived neurotrophic factor uses CREB and Egr3 to regulate NMDA receptor levels in cortical neurons
    Article Snippet: .. Fluorescent microscopy Primary neocortical cultured neurons were transfected with pDsRed2-F vector (Clontech) at DIV 7–8 to identify both plasma membranes and transfected neurons. ..

    Clinical Proteomics:

    Article Title: Brain-derived neurotrophic factor uses CREB and Egr3 to regulate NMDA receptor levels in cortical neurons
    Article Snippet: .. Primary neocortical cultured neurons were transfected with pDsRed2-F vector (Clontech) at DIV 7–8 to identify both plasma membranes and transfected neurons. ..

    Article Title: Brain-derived neurotrophic factor uses CREB and Egr3 to regulate NMDA receptor levels in cortical neurons
    Article Snippet: .. Fluorescent microscopy Primary neocortical cultured neurons were transfected with pDsRed2-F vector (Clontech) at DIV 7–8 to identify both plasma membranes and transfected neurons. ..

    Microscopy:

    Article Title: Brain-derived neurotrophic factor uses CREB and Egr3 to regulate NMDA receptor levels in cortical neurons
    Article Snippet: .. Fluorescent microscopy Primary neocortical cultured neurons were transfected with pDsRed2-F vector (Clontech) at DIV 7–8 to identify both plasma membranes and transfected neurons. ..



    Similar Products

    c pdsred2 c1 1nls d pdsred2 c1 s100a10 wt e pdsred2 c1 s100a10 1nls f control g pdsred2 c1 wt  (TaKaRa)
    95
    TaKaRa c pdsred2 c1 1nls d pdsred2 c1 s100a10 wt e pdsred2 c1 s100a10 1nls f control g pdsred2 c1 wt
    Map and MCS region of <t>pDsRed2-C1-wt.</t> a Vector of pDsRed2-C1-wt (Clontech), b pDsRed2-C1-wt MCS region (Clontech). c MCS regions of pDsRed2-C1-wt and <t>pDsRed2-C1-1NLS.</t> The NLS sequence was inserted between positions 1335 and 1339 in the sequence of pDsRed2-C1-wt (Clontech), and the CCG sequence was mutated to the CGC sequence. (a) was made by plasMapper 2.0 (https://wishart.biology.ualberta.ca/PlasMapper/index.html). For more information of pDsRed2-C1-wt, visit Clontech site (https://www.takarabio.com/assets/documents/Vector%20Documents/PT3603-5.pdf)
    C Pdsred2 C1 1nls D Pdsred2 C1 S100a10 Wt E Pdsred2 C1 S100a10 1nls F Control G Pdsred2 C1 Wt, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdsred2+f+vector/pDsRed2-C1+Vector/pmc06534648-171-22-32
    Average 95 stars, based on 1 article reviews
    c pdsred2 c1 1nls d pdsred2 c1 s100a10 wt e pdsred2 c1 s100a10 1nls f control g pdsred2 c1 wt - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    pdsred2 f vector  (TaKaRa)
    95
    TaKaRa pdsred2 f vector
    Map and MCS region of <t>pDsRed2-C1-wt.</t> a Vector of pDsRed2-C1-wt (Clontech), b pDsRed2-C1-wt MCS region (Clontech). c MCS regions of pDsRed2-C1-wt and <t>pDsRed2-C1-1NLS.</t> The NLS sequence was inserted between positions 1335 and 1339 in the sequence of pDsRed2-C1-wt (Clontech), and the CCG sequence was mutated to the CGC sequence. (a) was made by plasMapper 2.0 (https://wishart.biology.ualberta.ca/PlasMapper/index.html). For more information of pDsRed2-C1-wt, visit Clontech site (https://www.takarabio.com/assets/documents/Vector%20Documents/PT3603-5.pdf)
    Pdsred2 F Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdsred2+f+vector/pDsRed2-Bid+Vector/pmc05116917-108-7-9
    Average 95 stars, based on 1 article reviews
    pdsred2 f vector - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    Map and MCS region of pDsRed2-C1-wt. a Vector of pDsRed2-C1-wt (Clontech), b pDsRed2-C1-wt MCS region (Clontech). c MCS regions of pDsRed2-C1-wt and pDsRed2-C1-1NLS. The NLS sequence was inserted between positions 1335 and 1339 in the sequence of pDsRed2-C1-wt (Clontech), and the CCG sequence was mutated to the CGC sequence. (a) was made by plasMapper 2.0 (https://wishart.biology.ualberta.ca/PlasMapper/index.html). For more information of pDsRed2-C1-wt, visit Clontech site (https://www.takarabio.com/assets/documents/Vector%20Documents/PT3603-5.pdf)

    Journal: 3 Biotech

    Article Title: Development of a novel DsRed-NLS vector with a monopartite classical nuclear localization signal

    doi: 10.1007/s13205-019-1770-0

    Figure Lengend Snippet: Map and MCS region of pDsRed2-C1-wt. a Vector of pDsRed2-C1-wt (Clontech), b pDsRed2-C1-wt MCS region (Clontech). c MCS regions of pDsRed2-C1-wt and pDsRed2-C1-1NLS. The NLS sequence was inserted between positions 1335 and 1339 in the sequence of pDsRed2-C1-wt (Clontech), and the CCG sequence was mutated to the CGC sequence. (a) was made by plasMapper 2.0 (https://wishart.biology.ualberta.ca/PlasMapper/index.html). For more information of pDsRed2-C1-wt, visit Clontech site (https://www.takarabio.com/assets/documents/Vector%20Documents/PT3603-5.pdf)

    Article Snippet: Vectors transfected in COS1 ( a – e ) and COS7 ( f – j ) cells. a Control b pDsRed2-C1-wt (Clontech) c pDsRed2-C1-1NLS d pDsRed2-C1-S100A10-wt e pDsRed2-C1-S100A10-1NLS f control g pDsRed2-C1-wt (Clontech) h pDsRed2-C1-1NLS i pDsRed2-C1-S100A10-wt j pDsRed2-C1-S100A10-1NLS.

    Techniques: Plasmid Preparation, Sequencing

    Primers used for subcloning and site-directed mutagenesis

    Journal: 3 Biotech

    Article Title: Development of a novel DsRed-NLS vector with a monopartite classical nuclear localization signal

    doi: 10.1007/s13205-019-1770-0

    Figure Lengend Snippet: Primers used for subcloning and site-directed mutagenesis

    Article Snippet: Vectors transfected in COS1 ( a – e ) and COS7 ( f – j ) cells. a Control b pDsRed2-C1-wt (Clontech) c pDsRed2-C1-1NLS d pDsRed2-C1-S100A10-wt e pDsRed2-C1-S100A10-1NLS f control g pDsRed2-C1-wt (Clontech) h pDsRed2-C1-1NLS i pDsRed2-C1-S100A10-wt j pDsRed2-C1-S100A10-1NLS.

    Techniques: Subcloning, Construct

    Confocal microscopy experiments. Vectors transfected in COS1 (a–e) and COS7 (f–j) cells. a Control b pDsRed2-C1-wt (Clontech) c pDsRed2-C1-1NLS d pDsRed2-C1-S100A10-wt e pDsRed2-C1-S100A10-1NLS f control g pDsRed2-C1-wt (Clontech) h pDsRed2-C1-1NLS i pDsRed2-C1-S100A10-wt j pDsRed2-C1-S100A10-1NLS. Controls (a) and (f) correspond to non-treated COS1 and COS7 cells

    Journal: 3 Biotech

    Article Title: Development of a novel DsRed-NLS vector with a monopartite classical nuclear localization signal

    doi: 10.1007/s13205-019-1770-0

    Figure Lengend Snippet: Confocal microscopy experiments. Vectors transfected in COS1 (a–e) and COS7 (f–j) cells. a Control b pDsRed2-C1-wt (Clontech) c pDsRed2-C1-1NLS d pDsRed2-C1-S100A10-wt e pDsRed2-C1-S100A10-1NLS f control g pDsRed2-C1-wt (Clontech) h pDsRed2-C1-1NLS i pDsRed2-C1-S100A10-wt j pDsRed2-C1-S100A10-1NLS. Controls (a) and (f) correspond to non-treated COS1 and COS7 cells

    Article Snippet: Vectors transfected in COS1 ( a – e ) and COS7 ( f – j ) cells. a Control b pDsRed2-C1-wt (Clontech) c pDsRed2-C1-1NLS d pDsRed2-C1-S100A10-wt e pDsRed2-C1-S100A10-1NLS f control g pDsRed2-C1-wt (Clontech) h pDsRed2-C1-1NLS i pDsRed2-C1-S100A10-wt j pDsRed2-C1-S100A10-1NLS.

    Techniques: Confocal Microscopy, Transfection

    Fluorescence density analysis. Vectors transfected in COS1 (a–e) and COS7 (f–j) cells. a Control b pDsRed2-C1-wt (Clontech) c pDsRed2-C1-1NLS d pDsRed2-C1-S100A10-wt e pDsRed2-C1-S100A10-1NLS f control g pDsRed2-C1-wt (Clontech) h pDsRed2-C1-1NLS i pDsRed2-C1-S100A10-wt j pDsRed2-C1-S100A10-1NLS. Blue line represents the fluorescence intensity in the nucleus and red represents the fluorescence intensity of DsRed. Controls (a) and (f) correspond to non-treated COS1 and COS7 cells

    Journal: 3 Biotech

    Article Title: Development of a novel DsRed-NLS vector with a monopartite classical nuclear localization signal

    doi: 10.1007/s13205-019-1770-0

    Figure Lengend Snippet: Fluorescence density analysis. Vectors transfected in COS1 (a–e) and COS7 (f–j) cells. a Control b pDsRed2-C1-wt (Clontech) c pDsRed2-C1-1NLS d pDsRed2-C1-S100A10-wt e pDsRed2-C1-S100A10-1NLS f control g pDsRed2-C1-wt (Clontech) h pDsRed2-C1-1NLS i pDsRed2-C1-S100A10-wt j pDsRed2-C1-S100A10-1NLS. Blue line represents the fluorescence intensity in the nucleus and red represents the fluorescence intensity of DsRed. Controls (a) and (f) correspond to non-treated COS1 and COS7 cells

    Article Snippet: Vectors transfected in COS1 ( a – e ) and COS7 ( f – j ) cells. a Control b pDsRed2-C1-wt (Clontech) c pDsRed2-C1-1NLS d pDsRed2-C1-S100A10-wt e pDsRed2-C1-S100A10-1NLS f control g pDsRed2-C1-wt (Clontech) h pDsRed2-C1-1NLS i pDsRed2-C1-S100A10-wt j pDsRed2-C1-S100A10-1NLS.

    Techniques: Fluorescence, Transfection

    Immunoprecipitation of modified pDsRed2-C1-1NLS and pDsRed2-C1-S100A10-1NLS. Immunoprecipitation was performed using COS1 and COS7 cell lines. pDsRed2-C1-wt (Clontech), pDsRed2-C1-1NLS, pDsRed2-C1-S100A10-wt, and pDsRed2-C1-S100A10-1NLS vectors were transfected into each cell line for 24 h. Cells were lysed after transfection and immunoprecipitation was performed in the lysate using DsRed, karyopherin α2/β2 antibodies, and protein A/G plus-agarose (Santa Cruz). Samples that transfected with pDsRed2-C1-wt (Clontech), pDsRed2-C1-1NLS, pDsRed2-C1-S100A10-wt, pDsRed2-C1-S100A10-1NLS were identified using antibodies that detected karyopherin α2/β2 protein. Samples evaluated for karyopherin α2/β2 were confirmed by western blotting using antibodies for DsRed protein. Anti-β-actin (C4) antibody (Santa Cruz) was used to detect a reference protein in whole cell lysates prior to immunoprecipitation

    Journal: 3 Biotech

    Article Title: Development of a novel DsRed-NLS vector with a monopartite classical nuclear localization signal

    doi: 10.1007/s13205-019-1770-0

    Figure Lengend Snippet: Immunoprecipitation of modified pDsRed2-C1-1NLS and pDsRed2-C1-S100A10-1NLS. Immunoprecipitation was performed using COS1 and COS7 cell lines. pDsRed2-C1-wt (Clontech), pDsRed2-C1-1NLS, pDsRed2-C1-S100A10-wt, and pDsRed2-C1-S100A10-1NLS vectors were transfected into each cell line for 24 h. Cells were lysed after transfection and immunoprecipitation was performed in the lysate using DsRed, karyopherin α2/β2 antibodies, and protein A/G plus-agarose (Santa Cruz). Samples that transfected with pDsRed2-C1-wt (Clontech), pDsRed2-C1-1NLS, pDsRed2-C1-S100A10-wt, pDsRed2-C1-S100A10-1NLS were identified using antibodies that detected karyopherin α2/β2 protein. Samples evaluated for karyopherin α2/β2 were confirmed by western blotting using antibodies for DsRed protein. Anti-β-actin (C4) antibody (Santa Cruz) was used to detect a reference protein in whole cell lysates prior to immunoprecipitation

    Article Snippet: Vectors transfected in COS1 ( a – e ) and COS7 ( f – j ) cells. a Control b pDsRed2-C1-wt (Clontech) c pDsRed2-C1-1NLS d pDsRed2-C1-S100A10-wt e pDsRed2-C1-S100A10-1NLS f control g pDsRed2-C1-wt (Clontech) h pDsRed2-C1-1NLS i pDsRed2-C1-S100A10-wt j pDsRed2-C1-S100A10-1NLS.

    Techniques: Immunoprecipitation, Modification, Transfection, Western Blot